HIV cannot be transmitted through urine. In fact, only 
antibodies (produced by the immune system to combat infection, but 
aren’t infectious themselves) are present in urine. HIV also cannot be 
transmitted through tears, sweat, or faeces, nor can it be spread via 
toilet seats, air, water, insects, casual contact, or drinking 
fountains. The virus can only be transmitted through blood, semen and 
pre-seminal fluid, vaginal and cervical secretions, and breast milk. 
 Although HIV cannot be transmitted through urine, a pee sample can be 
used to test for the presence of HIV in the body. An enzyme immunoassay 
detects antibodies using urine, oral fluid, or blood as samples. A 
positive enzyme immunoassay test is then followed up by a confirmatory 
follow-up test.

 
